Yuan Tian

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Questions related from Yuan Tian

Asking for help: My pGEX transformed BL21 colonies from a LB plate didn't grow in LB medium.Why?

Hi, I am Yuan and I am new to this experiment. I aim to express a protein by pGEX construct (Ampiciline), I used BL21 to do transformation, and I got some colonies on the plate(LB Amp) but when I...

16 August 2023 6,417 1 View

Any ideas on how to get rid of the blue overexposed marker on WB membrane image scanned by LICOR-Oddesy?

I am using the LI-COR-Odessy system to image the membrane of western blots. Sometimes I can see blue markers covered on my bands. Maybe it is the indicator of overexposed bands. Does anyone know...

14 March 2023 482 3 View