How to phosphorylated the regulator PhoB(Asp) in E.coli and separated it by phos-tag SDS-PAGE?

At first, 10 μM N-His6-PhoB with 16 extra amino acids, 50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 10 mM MgCl2 with 50 mM lithium potassium acetyl phosphate were reacted at 37℃, remained 90 min. After...

15 March 2025 3,017 0 View