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By detecting the Km and Vmax values of the crude and pure enzyme, it was found that the Vmax of the pure enzyme remained the same, but the Km value increased. That is, the Km of the pure enzyme...
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The total protein content of the sample is sufficient. Although the saturated concentration of ammonium sulfate was added to 85%, less than 10% of the protein was precipitated. Would the presence...
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We use AKTA prime plus system. The machine shows high pressure after running for a while even at low flow rates. We checked any tubing and found a blockage when the water flowed through the flow...
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I used a kind of medium containing vegetable oil to induce bacterial protein, then ammonium sulfate fractionation to concentrate the protein. After resuspending the protein in the buffer, oil...
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