Yu Wen Cheng

4 Questions 3 Answers 0 Followers

Questions related from Yu Wen Cheng

How to interpret enzyme kinetic parameters?

By detecting the Km and Vmax values of the crude and pure enzyme, it was found that the Vmax of the pure enzyme remained the same, but the Km value increased. That is, the Km of the pure enzyme...

16 December 2022 4,974 3 View

Reasons for the poor effect of ammonium sulfate on protein precipitation?

The total protein content of the sample is sufficient. Although the saturated concentration of ammonium sulfate was added to 85%, less than 10% of the protein was precipitated. Would the presence...

29 May 2022 7,801 3 View

How to solve the problem of high pressure caused by blockage on FPLC/flow restrictor?

We use AKTA prime plus system. The machine shows high pressure after running for a while even at low flow rates. We checked any tubing and found a blockage when the water flowed through the flow...

25 February 2022 893 4 View

Is it possible to remove vegetable oil through a dialysis membrane?

I used a kind of medium containing vegetable oil to induce bacterial protein, then ammonium sulfate fractionation to concentrate the protein. After resuspending the protein in the buffer, oil...

01 December 2021 7,448 4 View