Is it possible to do F-actin staining with fixed HepG2 and HeLa cells without permeabilisation?

The experiment involves uptake of a fluorescent compound and confocal imaging. Attempts to fix the cells with 4% PFA and staining 1:50 with Alexa Fluor 633-conjugated phalloidin in accordance with...

23 April 2016 258 3 View