Signal in the no-RT control in ss-RT-qPCR for one gene only (HIS3) with no signal in the no-RT control of the internal control (ACT1). Any thoughts?

Hello, I am experiencing a weird problem with strand-specific RT-qPCR. I keep getting a lot of signal in the "no-RT control" for the gene of interest (HIS3) while I am getting nothing in the...

18 July 2018 4,585 0 View