Any advice on why I cannot calculate protein encapsulation in PLGA nanoparticles?

my blank NP absorbency in BCA method is higher than NP contain protein. it s maybe because PVA in blank NP or i dont have protein encapsulation. i confuse, also i used NaoH 1M and urea 10M+ SDS 2%...

29 November 2015 9,542 4 View