Yoshus Hoosier

3 Questions 10 Answers 0 Followers

Questions related from Yoshus Hoosier

What antifoam do you use and how do you use it?

I have been using Antifoam B for E.coli fermentation. I have used this without a problem in the past. I use a 5% solution that is pumped into the fermenter on a need basis as detected by the...

09 December 2013 7,851 4 View

XbaI/SpeI Digestion

Has anyone ever done this digestion. The digestion efficiency seems very low. The plasmid is very bright and the insert looks very faint on the gel? There is no dam methylation problem btw. Anyone...

10 May 2012 433 8 View

Bacterial P450 Reductase System

I am working on a bacterial P450 enzyme. I am wondering whether anyone knows of a P450-reductase that can be purchased or the general way to proceed to see activity. I have the enzyme and the...

01 March 2012 3,165 3 View