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Questions related from Yoshus Hoosier
I have been using Antifoam B for E.coli fermentation. I have used this without a problem in the past. I use a 5% solution that is pumped into the fermenter on a need basis as detected by the...
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I am interested in understanding on a very fundamental level why certain chemicals are used in growth of actinobacteria. For example, corn steep liquor, meat extract, dextrin, CaCO3 etc. This...
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Has anyone ever done this digestion. The digestion efficiency seems very low. The plasmid is very bright and the insert looks very faint on the gel? There is no dam methylation problem btw. Anyone...
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I am working on a bacterial P450 enzyme. I am wondering whether anyone knows of a P450-reductase that can be purchased or the general way to proceed to see activity. I have the enzyme and the...
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