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I wonder if recombinant transmembrane proteins need to be modified, for instance fusing a gag protein recognized sequence to the intracellular region, such that the TM proteins can be recognized...
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I have transfected two plasmids contain GFP1-10 and GFP11 into HEK293T. According to the papers, the two GFP splits express in the cell should able to form the entire GFP which will fluorescent....
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