What is the best reliable primers set /protocol for SYBR Green I based quantitative detection of Dengue and nested PCR for sero-typing?

Presently I'm using Lanciotti et al., protocol for detection of Dengue using SYBR green1 PCR and I need to extend it for quantification of copy number / viral load in patient serum samples. I...

20 September 2018 8,873 1 View