Yogesh Sharma

4 Questions 11 Answers 0 Followers

Questions related from Yogesh Sharma

Protein structure in united atom or all atom forcefield?

Hi, I have been carrying out simulation of a protein in presence of a number of small molecules and i observed molecules are getting tethered to COO side chain of polar charged surface residues. I...

02 February 2020 1,804 6 View

What are the best tools for aquaporin structure predictions?

Hi everyone I am trying to predict 3D structures of aquaporins for further simulation studies. I have used swissmodel itasser etc. for structure prediction. Although basic characteristic...

02 February 2020 2,029 16 View

Dangling bonds in protein terminals?

I am performing protein ligand simulations in Gromacs. In md simulations how can one fix these dangling movements of residues near protein terminals? I think this will interfere in my RMSD or...

01 January 2020 9,944 3 View

StrepII tagged antibody is showing transgene equivalent band in wild type strain. How?

hey guys I am using strep II tag for detection of an heterologously expressed protein. On western analysis I am getting exact band even in non transgenic wild type strain. I have checked my wild...

04 April 2019 285 3 View