Yizhe Zhang

2 Questions 3 Answers 0 Followers

Questions related from Yizhe Zhang

How can I Insert a long-sized fragment into the mammalian cell genome?

Hello everyone. I am interested in inserting a large-sized fragment (~20-30kb) into the genome of mammalian cells. What methods can I employ to achieve this? Additionally, how can I obtain the...

09 September 2023 4,304 1 View

How can I get single cell suspension of HepG2?

I want to isolate single cell clones of HepG2 from the pool using limiting dilution cloning (LDC) in 96-well plates. But I found that it's hard to get single cell suspension of HepG2 (at least 30%...

22 May 2023 503 5 View