Yiyu Meng

6 Questions 7 Answers 0 Followers

Questions related from Yiyu Meng

How to make the cells less sticky for flow FISH?

I am currently optimising a flow FISH protocol for an archaea species. The cells are fixed with and final concentration of 50% (v/v) ethanol before FISH hybridisation. The FISH protocol is carried...

22 February 2018 2,413 1 View

What is the current standard when dealing with singletons and rare sequences (OTUs) in NGS of PCR amplicons?

Hi all, I would like to get some suggestions and clarification about dealing with singletons in NGS of PCR amplicons. in the mothur SOP, the QC steps include the following: initial...

23 November 2016 6,618 4 View

Will the topology of a phylogenetic tree change after removing a number of sequences and re-run the treeing method?

Hello, suppose I have constructed a phylogenetic tree with all OTU sequences and reference sequences. I now want to have a tree containing only the OTU sequences. Is it a good practical to remove...

11 June 2016 8,438 4 View

What is the assumption for Brown Forsythe test and Welch test?

Hi all, Could anyone tell me what is the assumption for Brown Forsythe test and Welch test. This tests can be found in the ANOVA option in SPSS and can be used when homogeneity of variance of the...

12 May 2015 1,682 23 View

Does anyone knows what statistical analysis is used for the pairwise comparison in Kruskal-Wallis test, in SPSS version 22?

I found out that there is a handy option in the Kruskal-Wallis test, to do pairwise comparison between groups, in SPSS version 22. Anyone knows what statistics does it use?

06 May 2015 4,191 17 View

Will PCR amplify efficiently if the primer has one base mismatch at the 3' end?

I am recently using a primer set to amplify some DNA 16S rRNA from soil. One primer has one base pair mismatch at the 3' end. I would like to know how will that one base mis-match at the 3' end...

23 June 2014 9,956 7 View