Yishi Liu

3 Questions 3 Answers 0 Followers

Questions related from Yishi Liu

Can shRNA or SiRNA knockdown a highly expressed gene as efficiently as genes that are expressed at a lower level?

This might be a naive question, but I just wonder if there are more transcripts in the cells, would it be harder for the knockdown mechanism to degrade all these transcripts? Imaging for a same...

15 April 2015 4,158 4 View

Can you resuspend lentivirus pellets in culture media other than PBS?

I was just wondering if one could resuspend the lentivirus pellet in whatever culture medium, instead of PBS. In this way maybe the final concentration of virus can get higher when later...

27 October 2014 9,060 7 View

What is the best endotoxin-free maxi/midi prep kit?

I am trying to nucleofect/electroporate my T cell clones. These clones seem to be very sensitive to endotoxin in the plasmid preparation. I wonder if someone could give me some recommendation of...

03 August 2014 9,558 6 View