Why can I not lysis nuclei following published chromosome conformation capture (3C) protocol?

I tried to apply 3C experiment in NIH/3T3 cell line. NIH/3T3 is a Mouse Embryonic Fibroblast cell line. 1*10^7 cells were used for 3C experiment. However, by adding 0.3% sds in 1* NEB buffer 2.1,...

10 October 2015 6,782 3 View