Yinlei Bai

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How to examine the constitutive NF-κB activation by Western or RT-PCR? Are negative control needed?

I want to determine the constitutive NF-kappaB in MM cell lines by western or/and RT-PCR but I don't know the criteria. Are negative/positive control cell lines needed to see which is...

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Could NAIVE CD4 T cells be cultured in medium with only anti-CD3?

Hi, I sorted NAIVE CD4 T cells from mouse spleen and lymph nodes, and cultured cells in medium (RPMI1640 + Pen/Strep + 10%FBS + beta-mercaptoethanol + Glutamax-I + Sodium Pyruvate + HEPES) in a...

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