Yinlei Bai

4 Questions 8 Answers 0 Followers

Questions related from Yinlei Bai

Protocol for isolate leukocytes from mouse stomach?

Hi, everyone, I want to isolate CD45 +ve and -ve cells from mouse stomatch. I tried once. Stomach was cutted into peices as small as I can, and then digested in RPMI1640 medium containing 20mM...

21 November 2019 5,155 1 View

Bad performance of RQ-PCR standard curve constructed by plasmid

Hi guys! Recently I encountered a problem about the Albumin (ALB) standard curve which was used for normalizing my samples. The ALB standard curve was constructed by serial dilutions of plasmid...

26 July 2017 8,890 3 View

How to examine the constitutive NF-κB activation by Western or RT-PCR? Are negative control needed?

I want to determine the constitutive NF-kappaB in MM cell lines by western or/and RT-PCR but I don't know the criteria. Are negative/positive control cell lines needed to see which is...

07 January 2014 2,369 2 View

Could NAIVE CD4 T cells be cultured in medium with only anti-CD3?

Hi, I sorted NAIVE CD4 T cells from mouse spleen and lymph nodes, and cultured cells in medium (RPMI1640 + Pen/Strep + 10%FBS + beta-mercaptoethanol + Glutamax-I + Sodium Pyruvate + HEPES) in a...

01 January 1970 2,291 11 View