Yingying Law

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Questions related from Yingying Law

How to perform a low volume protein precipitation?

I have a start volume of 530ul crosslinked protein sample. I want to precipitate it. The Methanol/Chloroform and acetone methods require quite a bit of volume for precipitation, making it so that...

15 March 2021 7,798 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

25 February 2021 6,042 3 View