What could be the reason for the GPCR membrane protein exhibiting an unexpectedly lower molecular weight on SDS-PAGE?

After purifying my GPCR, I found that the protein, which is expected to be around 44 kDa, appears to be less than 40 kDa on SDS-PAGE. What could be the possible reasons for this unexpected lower...

19 October 2024 3,263 3 View