Yinghui Li

5 Questions 9 Answers 0 Followers

Questions related from Yinghui Li

A detailed protocol for lysing cells for luminescence ATP assay?

I'm using a luminescence kit from Thermo Fisher to do ATP assays for attached cells in 96 well plates. I'm looking for a easy to lyse the cells. I saw some people recommend to lyse the cells in...

05 May 2019 6,612 2 View

Will hoechst affect the Bio-Rad DC protein quantification?

I'm going to do the cell counting with Hoechst 33342 for nuclear staining, and then go to protein quantification using Bio-Rad DC protein assay reagent. Not sure if the blue fluorescence of...

03 March 2019 4,605 2 View

Can Lysotracker Red be used for fixed tissues?

I'm going to use Lysotracker for frozen sections to assess autophagy. Now I have some questions about it. 1. I read someone's work indicating that Lysotracker Red but not Green can be used for...

04 April 2018 9,094 3 View

Any recommendation for gradient gel for 30 KD protein?

I am working the purification of protein for mass spec. I want separate some bands around 30 KDa with precast gel. I used Bolt™ 4-12% Bis-Tris Plus Gels (Invitrogen) in MOPS buffer. But it didn't...

03 March 2018 6,602 5 View

Why the plasmid precipitated after adding to Opti-MEM with p3000?

I was doing transfection of N2A cells in 15 cm dishes with Lipofectamine 3000 (46 ug plasmid/dish). I found a strange phenomenon that I never noticed before. When I add the plasmid to Opti-MEM...

01 January 2018 7,534 10 View