Ying Lam Tang

4 Questions 1 Answers 0 Followers

Questions related from Ying Lam Tang

Why did my collagen I fail to form gel?

I would like to test the gelation conditions before I do 3D culture in collagen. Here is my setup (everything on ice before plating on 24-well plate): 90uL of 3.5mg/mL rat tail type I...

30 January 2019 8,631 5 View

Why did my collagen I fail to form gel?

I would like to test the gelation conditions before I do 3D culture in collagen. Here is my setup (everything on ice before plating on 24-well plate): 90uL of 3.5mg/mL rat tail type I...

01 January 2019 3,465 5 View

How to make fusion protein with linker residues using overlap extension PCR?

I have a gene A (1400bp) in a plasmid and a gene B (300bp) in another plasmid. I would like to make a fusion protein of A-GS linker-B using overlap extension PCR cloning. How should I design the...

09 September 2018 3,416 4 View

How does ExoSAP selectively destroy primers without PCR product loss?

11 July 2017 3,562 2 View