Ying Ding

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Questions related from Ying Ding

Aberrant protein after crispr/cas9?

Hi All, I used Crispr/cas9 to knockout CISH in T cells. The T7EI assay showed the gene editing. However, when I used the Western blot to check the protein expression, I found the the bands with...

11 November 2018 9,946 5 View

Why are the cells with high knockdown efficiency not the brightest cells ?

I inserted shRNA in pLK0.3G (a lentiviral vector with GFP as a reporter) to generate a shRNA plasmid and produce lentivirus for transduction in Tregs. After 3 days transduction, Tregs were...

05 May 2017 7,699 2 View

What's the order of the shRNA sequence in the plasmid?

I want to insert shRNA sequence to a vector with U6 promoter. What's the order of shRNA sequence? 5'-U6-sense-loop-antisense-terminator-3' or 5'-U6-antisense-loop-sense-terminator-3'? Is the...

08 August 2016 4,569 3 View