Yi Xu

6 Questions 27 Answers 0 Followers

Questions related from Yi Xu

What is size of primer dimer?

I am just wondering what is the exact size of PCR primer dimer? Supposing that both of my primers are ~25bp, the PCR product should be no more than 50bp, but why I can see a larger size with more...

08 August 2017 3,607 18 View

How can I extract the detergent-insoluable protein from membrane proteins?

Do you know how I can extract the detergent-insoluable protein from membrane proteins which will be used for co-immunoprecipitation assay? Thanks.

04 April 2015 7,218 10 View

How can I quantify bacterial DNA genome level from a single insect?

Hi, I have to measure a bacterial DNA level in single insect level. Do you have any idea about how to quantify them? By insect weight or instar stage?

03 March 2015 9,778 12 View

How do you filter specific small RNAs from two different small RNA libraries?

12 December 2013 8,375 1 View

Why use Oligo-(dT) primer for reverse transcription and qRT-PCR to detect relative gene expression levels of virus-encoded transcript?

When the virus messenge RNA do not contain a poly(A).

12 December 2013 1,142 9 View

How to detect the gene expression level of two homologous gene with few amino-acid mutants?

These two genes share almost the same sequence and have only three to four different amino acids.

09 September 2013 3,141 5 View