Band on DNA agarose gel is not clear? Which part is supercoil?

I run some plasmid samples with 1% agarose gel (like the picture shown), and the band is not clear, I use 5ul DNA sample with 1ul dye in each wall, 120V for 40 minutes, 1 and 6 are ladder, the...

13 April 2023 6,352 3 View