Yi-Ting Tsai

3 Questions 2 Answers 0 Followers

Questions related from Yi-Ting Tsai

Can I use T-overhang TOPO cloning vector for blunt end PCR product?

Hi, I know there are T-overhang and blunt end TOPO cloning vectors available from different vendors. Does anyone know if I can join blunt end PCR product with T-overhang TOPO vector? or I must...

31 May 2019 6,080 1 View

How to produce TOPO-vector for blunt end ligation?

Hi, I learn that using Vaccina virus Topoisomerase I can create sticky ends for A-overhang PCR product. But there are some commercial kits available for blunt end PCR product, like Invitrogen...

31 May 2019 2,160 0 View

Need I use western blot to confirm the untargeted LC-MS/MS result?

Hi, I had two samples (with or without treatment) sent for untargeted proteomics analysis through mass spectrometry. We found there is a change in amount of an enzyme. I want to know if I need...

04 May 2018 1,764 3 View