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So I am trying to design the qPCR primers for the mice Sp7. Mice Sp7 has 2 transcript variants, T1, T2, both of them have 2 exon. The T1 T2 only share the same sequence of the exon1/2, while they...
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I am preparing the TBST buffer for the western blot. The most common NaCl concentration is 150mM. However, I found tow the recipe from BIO-RAD with 500mM and 150mM NaCl concentration respectively....
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My hypothesis is Protein A and Protein B has interaction in cell. So I prefer to co-IP. About the strategies, which one should I choose? 1 Use beads covered with Anti-Protein A, pull the AB...
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