Does anyone have experience in TOPFLASH assay in HEK293T cells?

I did lipofectamine transfection in 12-well plates with 0.5M of cells and 0.8ug of TOPFLASH plasmid in each well. After 24h, I changed to media to serum-free media supplemented with 10uM...

21 October 2020 3,711 2 View