Yen-hsun Chang

4 Questions 3 Answers 0 Followers

Questions related from Yen-hsun Chang

For colocalization assay I used 2nd antibody of FITC and Alexa 568, but results look very weird?

hi, everyone Originally I use FITC 2nd antibody to label overexpressed protein while Alexa 568 to label a lysosome marker LAMP2, but the result was not good. Lysosome marker required enhancement...

03 July 2020 5,298 0 View

Serum starvation before transfection and cell doubling time?

Hi, everyone I have a simple question about timespan of serum starvation and transfection. Recently I work on U-2OS cells which have doubling time around 20 hours, and before I transfect U-2OS...

16 March 2020 3,450 4 View

How to separate dead lymphoblastoid cells from live ones?

Dead cells or cell debris are supposedly to be lighter than live cells, and therefore the most recommended procedure is to centrifuge and remove supernatants. Recently I used centrifugation at...

15 March 2019 1,649 2 View

Co-IP results in smearing bands

I’m wondering why in IP lanes, some bands are sharp, while some are smearing, even they are in the same lane. Originally I thought that is caused by non-specific binding to beads, but lane of IgG...

17 August 2017 8,241 1 View