Yelungka Thapa

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Western blotting or RT-PCR which has better significance?

HI I wanted to to know which data has better significance while comparing the results of RT PCR and Western blotting.

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Can I modify the steps in western blot? Has anyone had positive results in Cyclin D1?

Hi I did a western blotting in endometrial cancer cell lines for Cyclin D1 (1:1000, incubated over night, Santa Cruz) and used mouse as a secondary antibody (1:10,000, incubated for 4 hours). The...

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Need help with Western blotting?

HI I have been trying to work with Er alpha MC-20 from santa cruz. It fails to detect a band. I did follow the dilution factor provided by the protocol. Can anyone suggest me if they have...

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