Dear collegues, has anybody performed successful staining and fluorescent imaging of the cells grown on matrigel? Do you have technical advises?

The cells are EndoC-BH4. They are grown on matrigel, but they do not seem to be submerged into the gel. I tried to stain them with antibodies, phalloidin, and DAPI after paraformaldehyde fixation...

14 January 2019 4,725 3 View