Yazen Alnefeesi

3 Questions 8 Answers 0 Followers

Questions related from Yazen Alnefeesi

What should primers be dissolved in for high-fidelity PCR and downstream cloning of amplicons?

I'm amplifying a transgene using Pfu and plan to double-digest it with NotI and XhoI (same buffer) to ligate it into a plasmid. I've never done high-fidelity PCR and I'm not sure what the primers...

25 September 2021 6,555 3 View

DNA migrated backwards, and 3 tracking dyes moved as expected. Why did that happen?

Full disclosure: This was the third run using the same gel and running buffer (I added distilled water to compensate for evaporation). I know you're not supposed to reuse running buffer this much,...

09 January 2021 6,611 6 View

Can chloroform be effectively stabilized with isopropanol? And why does ethanol stabilization usually only involve 1% by mass?

The oxidation of chloroform is dangerous because it results in phosgene formation under exposure to UV light. Along with the minimization of UV exposure, this reaction is usually prevented with 1%...

31 December 2020 3,516 3 View