How can I make an elution to extract a protein on an IgG bead without washing off the protein?

I used GST-3C protease and Glutathione Sepharose 4B beads that were washed 3X with 3C buffer. Then everything was filtered through a SPIN-X centrifuge tube. When I ran the Coomassie Blue gel, I...

11 July 2018 2,173 1 View