How to improve intracellular cytokine staining for IL-4 and IL-10 in human PBMCs?

I'm trying to optimise an ICS protocol for assessing cytokine production in human PBMCs. While I have good staining for IFN-g and TNF-a using either PMA/ionomycin or CD3/CD28 stimulation, I am...

01 April 2014 7,596 7 View