Yating Li

2 Questions 2 Answers 0 Followers

Questions related from Yating Li

How can I reduce the leaky from a TRE (tet responding element)-containing plasmid?

I have two plasmid, one expressing tTA and the other one has a eGFP under TRE promoter. By right I would see GFP signal when both plasmids are in a cell. However when I transfected HEK293 cell...

12 December 2016 592 1 View

Does anyone have a problem doing stable transfection on a crispr knockout cell line?

I have stable EpH4 cell line with my gene of interest knockout and I'm planning to introduce the mutant gene into it using HEK293 cell, with hygromycin selection. Here are some problems I...

11 November 2015 5,584 1 View