Yasmeen Abuhadema

4 Questions 4 Answers 0 Followers

Questions related from Yasmeen Abuhadema

Why Non specific bands of PCR reaction appear while using specific primers for tobamoviruses?

i have done RNA extraction and cDNA for tobamovirus then i made a PCR reaction using primer pair Tob uni1/Tob uni2 according to the protocol in Letschert et al., 2002 ( annealing temp. 60C for 45s...

12 December 2019 7,482 3 View

Why my specific primer match to give cDNA but it does not work in PCR?

I work in plant virus and I use a specific primer to make the cDNA (I do not use oligo dT or hexamer) and it works giving me a sharp band (photo in attachment) But when I made the PCR using these...

08 August 2019 1,575 3 View

Why ELISA reading decrease with time!!! ??

I made ELISA test and read it after 50 min , the results made sense and it was OK But i reread it after 1h and 45 min but the results are less than the first time . Why this happened?

07 July 2018 6,022 3 View

How can i get a positive control for my PCR test on Alfalfa mosaic virus ?

I want to do a pcr test on Alfalfa mosaic virus and i need a postive control to confirm my results . So how can i get or buy it.

05 May 2018 8,735 2 View