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Hi everyone. I want to know how to to design a guide RNA for a particular exon or gene to generate knockout mouse model.
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whenever i buy new primers (100pM) they work fine at first dilution (10pM) as well as first 3-4 polymerase chain reactions. But when i use same or new dilutions (10pM) after 15- 20 days at the...
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My research is a lot dependent on bioinformatics as i am working on genetics of neurodevelopmental disorders. I am looking for a fully funded programme on WES/NGS data analysis or any online...
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When i run gradient PCR, it works well for 2-3 days, After that there is no amplification neither in same or other gradient ranges nor at temperature specified by first run. Is this issue with...
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