Yaser Rafiq Mir

4 Questions 7 Answers 0 Followers

Questions related from Yaser Rafiq Mir

I want to know how to design a guide RNA for a particular exon of a gene ?

Hi everyone. I want to know how to to design a guide RNA for a particular exon or gene to generate knockout mouse model.

23 December 2024 7,432 2 View

Please guide: Why my PCR lacks reproducibilty?

whenever i buy new primers (100pM) they work fine at first dilution (10pM) as well as first 3-4 polymerase chain reactions. But when i use same or new dilutions (10pM) after 15- 20 days at the...

21 July 2020 5,549 16 View

Whole exome sequencing data analysis training ?

My research is a lot dependent on bioinformatics as i am working on genetics of neurodevelopmental disorders. I am looking for a fully funded programme on WES/NGS data analysis or any online...

24 April 2020 6,615 0 View

What can be possible reason when there is no reproducibilty in PCR which ones functioned?

When i run gradient PCR, it works well for 2-3 days, After that there is no amplification neither in same or other gradient ranges nor at temperature specified by first run. Is this issue with...

18 March 2020 1,777 2 View