Yasemin Yoluc

3 Questions 4 Answers 0 Followers

Questions related from Yasemin Yoluc

How to grow yeast BY4743 with XX::MX4 - which medium components are needed?

I want to grow yeast (BY4743) with a certain knock out (deletion), the deletion cassette is KANMX4. I assume I have to use 200mcg/ml geneticin. But can anyone explain step by step how i have to...

20 November 2020 3,823 3 View

How can i keep my Phenol in water solution saturated?

I am using the hot phenol method to extract RNA. Usually, I am dissolving Phenol in water and keep it in the fridge ( 4°C ), i could see that it is a saturated solution because of the solid...

26 September 2019 6,006 5 View

Why isn't BY4741 growing anymore in freshly prepared YNB medium ?

Hallo, I'm using 1x YNB medium (0.67 g/ 100 mL) supplied with an Aminoacid mix. As my medium was going to finish I prepared new one, and set up two cultures, one with the "old" medium, i was...

14 February 2019 6,238 3 View