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I want to grow yeast (BY4743) with a certain knock out (deletion), the deletion cassette is KANMX4. I assume I have to use 200mcg/ml geneticin. But can anyone explain step by step how i have to...
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I am using the hot phenol method to extract RNA. Usually, I am dissolving Phenol in water and keep it in the fridge ( 4°C ), i could see that it is a saturated solution because of the solid...
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