Is there a way to eliminate non-spesific binding of HA-tagged proteins to M2 agarose?

Hi all, Im performing co-IP with Ha-tag protein lysates as prey. My problem is when i incubate M2 agarose + HA tagged protein lysate OR Ha-agarose+ flag-tagged protein lysate, my protein of...

22 January 2019 6,194 3 View