Yao Fu

3 Questions 7 Answers 0 Followers

Questions related from Yao Fu

How much antibody do I need for large volume sample in FACS?

Hi, I'd like to stain 10M cells with CD3 for cell sorting. The datasheet says 5ul/test, and typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). So does it mean I need a 50ul...

27 October 2023 5,291 2 View

Why do I get different results in 1-step-RT-PCR and 2-step-RT-PCR?

Here is my story: 1. I used total RNA which I extracted from cell as template, and used AccessQuick RT-PCR Kit (Promega A1702) to do 1-step-RT-PCR. Then I ran agarose gel and got target band. 2....

31 January 2018 8,486 2 View

Could anybody can explain why there are amplification curve and melting curve in the "no-reverse transcriptase" group in qPCR?

In my experiment, I want to test mRNA level by qPCR. First I isolated total RNA from mice cerebrum tissue and then did both 1-step qPCR and 2-step qPCR. I can clearly see there is a peak in...

19 December 2016 4,815 6 View