Yangqi Gu

4 Questions 3 Answers 0 Followers

Questions related from Yangqi Gu

Does TCEP cut vinyl bonds of hemes upon heating?

I recently noticed that when boiling my cytochrome sample with TCEP will show degradation product of my cytochromes (shown by extra lower molecular weight bands in heme staining). Since my sample...

01 July 2020 2,149 0 View

How to remove membrane contamination from protein sample?

Hi all, I’m trying to purify a filament protein (extracellular) and I have encountered a huge membrane contamination most likely due to the bleb from my bacteria. I have attempted sucrose...

06 December 2019 4,269 0 View

How to use mass-spec to detect covalent bonding of a dimer complex?

Hi all, I have a protein supposed to be around 20 kDa, but somehow on SDS-PAGE it runs at 40 kDa and previous literature also showed the same behavior and suggested a dimer complex. I am little...

31 July 2019 4,390 3 View

How to digest bacterial EPS at physiological temperature?

My bacterial biofilm tends to aggregate and agglutinate and I want to separate them at physiological temperature (25 - 30 C) in liquid. Any good suggestions of enzymes and preferably with...

29 January 2019 5,100 3 View