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Hello! I am doing a CRISPRi-screening. I prepared sequencing library by PCR amplification using a i5 common primer and i7 primers with single TruSeq index, and performed a paired sequencing on...
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I generated Hela-KRAB-dCas9 stable cell line, and to validate dCas9 activity, I introduced two sgRNA expressing vectors driven by either hU6 or mU6 promoter. I found that the mU6 promoter driven...
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