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Questions related from Yang Senshiki
http://www.ncbi.nlm.nih.gov/pubmed/21775964 My experiment to visualize dendritic spine is according to this article and it successed. However, recently I am doubting its mechanism. The vector I...
09 April 2016 5,679 3 View
We want to observe dendritic spine morphology on cortical neuron culture. So we transfected cells with plasmids expressing GFP. But, under 63x confocal GFP seems to aggregate into puncta. And the...
22 November 2015 4,288 3 View
I use phalloidin-iFluor 488 from Abcam(ab176753) to stain F-actin in cortical neuron cells. But it doesn't work. I think it should be easier to stain F-actin. What should I take care when I do the...
17 November 2015 6,388 6 View
Traditional golgi cox stain method can be used in brain tissue slice. However, I did not see in cell culture. Thus, does it need any modifications before applying to cell culture?
03 February 2015 2,884 1 View
