Yang Jiandong

4 Questions 6 Answers 0 Followers

Questions related from Yang Jiandong

How to do the FITC-dextran 4KDa for permeability testing?

Thank you for reading my questions! I want to do the permeability assay for the Caco-2 cells on the transwell-like structure. I have several questions: (1) What is the general concentration for...

01 June 2021 5,683 2 View

The difference between cell viability testing reagent-Calcein AM and Calcium Imaging?

Recently, I want to detect the cardiomyocyte cell viability including live cell staining, total cell nuclei staining and apoptosis cell staining. Also, in order to show the function of...

28 May 2018 6,141 4 View

Will gene background difference affects the cell-cell junction interaction and recognition?

Recently, I was studying the cell-cell junction interaction. I realized the junction protein of cells have selective connection property. For example, cadherin help selective cell–cell...

01 January 1970 1,335 0 View

Why some cells were not stained with Hoechst 33258?

Recently, I used the Hoechst 33258 to stain the nuclei of HepG2 cells. I did not fix the cells, because I want to combine the Hoechst staining with Calcein AM staining later. I thought the Hoechst...

01 January 1970 3,819 3 View