Will PCR product amplified using phusion HIFI DNA polymerase have 5' phosphate groups?

Hi Guys I am trying to clone my PCR product into PUC19 with blunt end ligation. I amplified my PCR product using phusion High-fidelity DNA polymerase (NEB). I cut the vector with SmaI and treated...

05 May 2016 6,167 3 View