Yamin Aung

3 Questions 4 Answers 0 Followers

Questions related from Yamin Aung

My cloning is not working. What could be the problem?

I am trying to clone a fusion PCR product in pcDNA3 vector. After double RE digestion, and direct ligation with the vector, bacteria transformation seems to be working. There is no colonies on the...

15 August 2019 1,406 3 View

How to extract mtDNA from isolated mitochondria?

Is there any kit or protocol we can use to extract mtDNA from isolated mitochondria instead of from tissues or cells? Also considering extraction from inner membrane and matrix fractions.

03 June 2019 9,119 3 View

What could possibly be the cause of my lactate assay not working?

I am using a lactate assay kit from Sigma Aldrich to measure lactate production in cell culture. But i am having trouble creating the standard curve firstly. I used 5mM lactate with 100mM NAD+, so...

24 May 2019 4,527 4 View