Yalena Amador-Cañizares

2 Questions 7 Answers 0 Followers

Questions related from Yalena Amador-Cañizares

Detection of Myc-tagged protein by anti-Myc but not by the specific antibody?

Hello everyone, I am using lentiviruses to overexpress proteins (Myc-tagged versions) of interest in hepatoma cells. After several rounds of selection with G418, all the untransduced cells were...

10 October 2017 4,309 6 View

Does anyone have experience with protein pulldowns using RNA baits?

I am doing protein pulldowns with biotinylated RNA baits. I start with a lysate of approximately 20x106 cells (one 150mm dish close to confluency). First I bind the biotinylated RNA molecule to...

08 August 2017 8,754 4 View