Has anyone experienced problems with BrdU staining the cytoplasm instead of the nucleus?

I'm doing BrdU staining in a cell line. I've treated the cells with 1.5 ug/ml of BrdU for 1h. Then I fixed them with 2% PFA for 15min, denaturated with HCl 4N for 10min RT, washes in PBS to...

29 September 2015 6,076 8 View