Yacoba Minnow

2 Questions 5 Answers 0 Followers

Questions related from Yacoba Minnow

Can anyone please explain to me how you calculate the amount of coupled enzyme to use in the coupled reaction?

I used a method as described in the reference below, I am not sure if I did it right. Any other suggestions and simpler methods are also welcome. Thank you Storer, A. C., & Cornish-Bowden, A....

06 June 2018 7,575 6 View

My 2uM inhibitor is faster than my zero inhibitor. Can anyone please tell me why?

In my assay, the substrate contributes a lot of DMSO, about 3.2% at a 100uM and contributes 0.8% making a total of 4% DMSO concentration in the assay. So we decided to make the DMSO concentration...

03 March 2016 2,744 6 View