Yacoba Minnow

2 Questions 6 Answers 0 Followers

Questions related from Yacoba Minnow

What is a gentle but efficient way to lyse bacterial cells to isolate recombinant protein?

Hello all, I am trying to isolate and purify my recombinant protein. I usually lyse the cells using sonication (15 sec on, 15 sec off for 7:30 mins; 2X) for all my other recombinant proteins. For...

25 April 2019 9,331 7 View

My 2uM inhibitor is faster than my zero inhibitor. Can anyone please tell me why?

In my assay, the substrate contributes a lot of DMSO, about 3.2% at a 100uM and contributes 0.8% making a total of 4% DMSO concentration in the assay. So we decided to make the DMSO concentration...

31 March 2016 712 5 View