Ya Yesh

5 Questions 12 Answers 0 Followers

Questions related from Ya Yesh

Is anyone familiar with low molecular protein separation in Gel Filtration columns?

Dear all, I have a protein (10Kda) which can form a complex with another ligand(400Da). The ligand is not soluble in water. It can be soluble in DMSO. I would like to check the complex...

09 September 2014 2,333 7 View

Can you troubleshoot my process of protein aggregation in gel filtration?

I am trying to check oligomerisation of my 10 kda protein & ligand in S75 tricon column. When I run the protein alone it elutes at 13ml (corresponds to 12 kda in standard protein marker). Here...

08 August 2014 7,428 15 View

Can anyone assist me with native gel troubleshooting?

I have question regarding Native gel (15%). My protein molecular weight is 13 kda and it supposed to form dimer when ligand is added.The ligand is around 500Da. When I run native gel, my protein...

08 August 2014 3,352 7 View

How can I find the possible mutation sites of the protein based on the sequence information?

As well how I can check the active site of an enzyme based on their sequence ? Thanks in advance for your suggestions.

08 August 2014 5,121 4 View

How do I check oligomers / dimers of the protein using Native Gel ?

I am new to protein chemistry, Can I know how do I check oligomers / dimers of the protein using Native Gel ? Thanks in advance.

08 August 2014 4,667 7 View