Ya Yesh

5 Questions 11 Answers 0 Followers

Questions related from Ya Yesh

Is anyone familiar with low molecular protein separation in Gel Filtration columns?

Dear all, I have a protein (10Kda) which can form a complex with another ligand(400Da). The ligand is not soluble in water. It can be soluble in DMSO. I would like to check the complex...

12 September 2014 184 7 View

Can anyone assist me with native gel troubleshooting?

I have question regarding Native gel (15%). My protein molecular weight is 13 kda and it supposed to form dimer when ligand is added.The ligand is around 500Da. When I run native gel, my protein...

30 August 2014 9,116 6 View

How do I check oligomers / dimers of the protein using Native Gel ?

I am new to protein chemistry, Can I know how do I check oligomers / dimers of the protein using Native Gel ? Thanks in advance.

28 August 2014 1,492 6 View

How can I find the possible mutation sites of the protein based on the sequence information?

As well how I can check the active site of an enzyme based on their sequence ? Thanks in advance for your suggestions.

27 August 2014 9,211 4 View

Can you troubleshoot my process of protein aggregation in gel filtration?

I am trying to check oligomerisation of my 10 kda protein & ligand in S75 tricon column. When I run the protein alone it elutes at 13ml (corresponds to 12 kda in standard protein marker). Here...

26 August 2014 9,005 15 View